Fig. 2

Effects of PFD on intracellular and membrane molecules characterizing differentiation and activity of SpA FLSs. a, c, and e Representative flow cytometry density plots of SpA FLSs untreated (UT) and stimulated (Stimulation) with or without pirfenidone (PFD). b, d, and f Column bar graph of percentage positive FLSs among all SpA FLSs untreated (UT) and stimulated (Stimulation) with or without PFD (n = 4). Data were normalized to stimulated cultures without PFD (ratio), log-transformed and analyzed with the paired t-test. Boxes and bars indicate median and IQR. a and b TGFβ stimulated FLSs were analyzed for intracellular αSMA expression. c and d IFNγ stimulated FLSs were analyzed for membrane HLA-DR expression. e and f TNFα stimulated FLSs were analyzed for membrane ICAM-1 expression. * p < 0.05. ** p < 0.01