Table 2 The amplification primer sequences
Gene name | GeneBank accession no. | Primer sequence (5’-3’) |
|---|---|---|
NLRP1 | NM_033004 | F-ATTGAGGGCAGGCAGCACAGAT |
| Â | Â | R-CTCCTTCAGGTTTCTGGTGACC |
NLRP2 | NM_017852 | F-CATTCTGCGTCAAGCACTGTCG |
| Â | Â | R-CCGTCCAGAAAGGAAGCATGTG |
NLRP3 | NM_004895 | F-GGACTGAAGCACCTGTTGTGCA |
| Â | Â | R-TCCTGAGTCTCCCAAGGCATTC |
NLRC4 | NM_021209 | F-AGGTCCCACAACTCGTCAAGCT |
| Â | Â | R-TGCTCACACGATTTCCCGCCAA |
NLRC5 | NM_032206 | F-AGTGGCTCTTCCGCTTGGACAT |
| Â | Â | R-CGGAACCCTAAGAACTTGGCTG |
NLRP12 | NM_144687 | F-CAGGCATGATGCTGCTTTGCGA |
| Â | Â | R-AGCACAGAAGCCATCTCCTGAC |
AIM2 | NM_004833 | F-GCTGCACCAAAAGTCTCTCCTC |
| Â | Â | R-CTGCTTGCCTTCTTGGGTCTCA |
CARD8 | NM_014959 | F-GTGTGGGATACTGAGGTGAAGC |
| Â | Â | R-TGTCCTGGAGATCATCGAGCAC |
IFI16 | NM_005531 | F-GATGCCTCCATCAACACCAAGC |
| Â | Â | R-CTGTTGCGTTCAGCACCATCAC |
Pyrin | NM_000243 | F-GCTGCTCTTCTGTGAGGATCAC |
| Â | Â | R-CTCAGCTTCTTCAGATGCTCCAG |
NAIP | NM_004536 | F-CCGAACAGGAACTGCTTCTCAC |
| Â | Â | R-CCACAGACAGTTCTTTCAGGCAC |
Caspase-1 | NM_033292 | F-GCTGAGGTTGACATCACAGGCA |
| Â | Â | R-TGCTGTCAGAGGTCTTGTGCTC |
Caspase-4 | NM_001225 | F-GGGATGAAGGAGCTACTTGAGG |
| Â | Â | R-CCAAGAATGTGCTGTCAGAGGAC |
Caspase-5 | NM_004347 | F-ACAACCGCAACTGCCTCAGTCT |
| Â | Â | R-GAATCTGCCTCCAGGTTCTCAG |
IL1beta | NM_000576 | F-CCACAGACCTTCCAGGAGAATG |
| Â | Â | R-GTGCAGTTCAGTGATCGTACAGG |
IL-18 | NM_001562 | F-GATAGCCAGCCTAGAGGTATGG |
| Â | Â | R-CCTTGATGTTATCAGGAGGATTCA |
GAPDH | NM_002046 | F-GTCTCCTCTGACTTCAACAGCG |
| Â | Â | R-ACCACCCTGTTGCTGTAGCCAA |