Table 2 The primer sequence, restriction enzymes, and fragment length of SNPs
SNP | Location | Primers (5’-3’) | Ta (◦C) | Enzyme | Genotypes and fragments (bp) |
|---|---|---|---|---|---|
rs187084 | 5’ flanking region | F: TCATTCAGCCTTCACTCAGAAA R: ACCTCCCACCCCAGATCT | 60° C | AflII | GG = 299 AA = 148 + 151 AG = 299 + 148 + 151 |
rs352140 | Exon 2 | F: GGCTGTGGATGTTGTTGTG R: GCAGCACCCTCAACTTCAC | 58° C | BstUI | TT = 360 CC = 132 + 228 CT = 360 + 132 + 228 |