Fig. 3

Effects of PFD on intracellular and membrane molecules characterizing differentiation and activity of SpA FLSs. a-b Images of membrane-based antibody array of SpA FLSs stimulated with TGFβ, TNFα, and IFNγ (TGFβ+TNFα+IFNγ) with or without pirfenidone (PFD). Black square marks MCP-1 and grey square marks YKL-40. c-d Column bar graph of MCP-1 and YKL-40 secretion by SpA FLSs untreated (UT) and stimulated with TGFβ, TNFα, IFNγ or all three cytokines (All) with or without PFD (n = 4). Data were normalized to untreated cultures without PFD (ratio), log-transformed and analyzed with the paired t-test. Boxes and bars indicate median and IQR. * p < 0.05. ** p < 0.01. *** p < 0.005